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mouse anti rat wnt 3a polyclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti rat wnt 3a polyclonal antibody
    Mouse Anti Rat Wnt 3a Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+rat+wnt+3a+polyclonal+antibody/Wnt3a+Antibody/pmc04768959-61-86-93
    Average 95 stars, based on 203 article reviews
    mouse anti rat wnt 3a polyclonal antibody - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis
    Article Snippet: The concentration of total protein was quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific, Inc., Wilmington, DE, USA). .. A total of 12 μ l total protein was loaded per lane and electrophoresed in 12% sodium dodecyl sulfate-polyacrylimide gel electrophoresis using a constant voltage of 80 V for 2 h. The proteins were then transferred onto a 0.45-nitrocellulose membrane (Hybond-ECL; GE Healthcare Life Sciences) at 350 mA for 90 min and blocked with 5% nonfat milk at room temperature for 2 h. The membranes were incubated overnight at 4°C with mouse anti-rat collagen II monoclonal antibody (mAb; 1:500; hm1062; Abgent, Inc., San Diego, CA, USA), mouse anti-rat Wnt-3a polyclonal antibody (1:50; 2391S; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-rat β-catenin mAb (1:50; sc-59737; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), mouse anti-rat Mmp-13 polyclonal antibody (1:50; bs668; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse anti-rat β-actin monoclonal antibody (1:3,000; CW0096; Abgent, Inc.). .. Subsequently the membranes were washed three times with Tris-buffered saline containing 5% Tween-20 (Sinopharm Chemical Reagent Co., Ltd.) for 5 min each, prior to incubation with horseradish peroxidase-conjugated goat anti-mouse polyclonal IgG (1:4,000; bs-0296p; BIOSS, Beijing, China) at 4°C for 2 h, immunoreactive proteins were visualized on an X-ray film (Sinopharm Chemical Reagent Co., Ltd.,) with an Enhanced Chemiluminescence kit (NEN Life Science Products, Inc., Boston, MA, USA).

    Membrane:

    Article Title: Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis
    Article Snippet: The concentration of total protein was quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific, Inc., Wilmington, DE, USA). .. A total of 12 μ l total protein was loaded per lane and electrophoresed in 12% sodium dodecyl sulfate-polyacrylimide gel electrophoresis using a constant voltage of 80 V for 2 h. The proteins were then transferred onto a 0.45-nitrocellulose membrane (Hybond-ECL; GE Healthcare Life Sciences) at 350 mA for 90 min and blocked with 5% nonfat milk at room temperature for 2 h. The membranes were incubated overnight at 4°C with mouse anti-rat collagen II monoclonal antibody (mAb; 1:500; hm1062; Abgent, Inc., San Diego, CA, USA), mouse anti-rat Wnt-3a polyclonal antibody (1:50; 2391S; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-rat β-catenin mAb (1:50; sc-59737; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), mouse anti-rat Mmp-13 polyclonal antibody (1:50; bs668; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse anti-rat β-actin monoclonal antibody (1:3,000; CW0096; Abgent, Inc.). .. Subsequently the membranes were washed three times with Tris-buffered saline containing 5% Tween-20 (Sinopharm Chemical Reagent Co., Ltd.) for 5 min each, prior to incubation with horseradish peroxidase-conjugated goat anti-mouse polyclonal IgG (1:4,000; bs-0296p; BIOSS, Beijing, China) at 4°C for 2 h, immunoreactive proteins were visualized on an X-ray film (Sinopharm Chemical Reagent Co., Ltd.,) with an Enhanced Chemiluminescence kit (NEN Life Science Products, Inc., Boston, MA, USA).

    Incubation:

    Article Title: Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis
    Article Snippet: The concentration of total protein was quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific, Inc., Wilmington, DE, USA). .. A total of 12 μ l total protein was loaded per lane and electrophoresed in 12% sodium dodecyl sulfate-polyacrylimide gel electrophoresis using a constant voltage of 80 V for 2 h. The proteins were then transferred onto a 0.45-nitrocellulose membrane (Hybond-ECL; GE Healthcare Life Sciences) at 350 mA for 90 min and blocked with 5% nonfat milk at room temperature for 2 h. The membranes were incubated overnight at 4°C with mouse anti-rat collagen II monoclonal antibody (mAb; 1:500; hm1062; Abgent, Inc., San Diego, CA, USA), mouse anti-rat Wnt-3a polyclonal antibody (1:50; 2391S; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-rat β-catenin mAb (1:50; sc-59737; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), mouse anti-rat Mmp-13 polyclonal antibody (1:50; bs668; Bioworld Technology, Inc., St. Louis Park, MN, USA) and mouse anti-rat β-actin monoclonal antibody (1:3,000; CW0096; Abgent, Inc.). .. Subsequently the membranes were washed three times with Tris-buffered saline containing 5% Tween-20 (Sinopharm Chemical Reagent Co., Ltd.) for 5 min each, prior to incubation with horseradish peroxidase-conjugated goat anti-mouse polyclonal IgG (1:4,000; bs-0296p; BIOSS, Beijing, China) at 4°C for 2 h, immunoreactive proteins were visualized on an X-ray film (Sinopharm Chemical Reagent Co., Ltd.,) with an Enhanced Chemiluminescence kit (NEN Life Science Products, Inc., Boston, MA, USA).



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    mouse anti rat wnt 3a polyclonal antibody  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc mouse anti rat wnt 3a polyclonal antibody
    Mouse Anti Rat Wnt 3a Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+rat+wnt+3a+polyclonal+antibody/Wnt3a+Antibody/pmc04768959-61-86-93
    Average 95 stars, based on 1 article reviews
    mouse anti rat wnt 3a polyclonal antibody - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier

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